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sequencing adapter for illumina nextera dna unique dual index  (Illumina Inc)


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    Structured Review

    Illumina Inc sequencing adapter for illumina nextera dna unique dual index
    Sequencing Adapter For Illumina Nextera Dna Unique Dual Index, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence+(b)/IDT+for+Illumina+Nextera+DNA+Unique+Dual+Indexes+Set+B/10__1007_slash_s41208___025___01001___3-68-6-9
    Average 97 stars, based on 34 article reviews
    sequencing adapter for illumina nextera dna unique dual index - by Bioz Stars, 2026-09
    97/100 stars

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    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Insight into the dynamic changes and relationship between organic acids, amino acids and microbial communities during the fermentation of highland barley wine
    Article Snippet: To create the low-alcoholic fermented wine with excellent nutritional properties, this study explored the dynamic changes in organic acids, amino acids and microbial communities during the fermentation of highland barley wine (HBW) and their correlation.. A total of 7 organic acids and 16 amino acids were detected in HBW at different fermentation time.. Partial least squares-discriminant analysis (PLS-DA) showed that lactic acid, acetic acid, arginine, proline and glutamate were the characteristic metabolites.

    Article Title: The DNA methylation landscape of primary triple-negative breast cancer
    Article Snippet: RNA-sequencing SCAN-B RNA-sequencing was performed by Illumina stranded TruSeq mRNA protocol, either implemented on KingFisher or on the Illumina NeoPrep system.

    Sequencing:

    Article Title: Comparison of nanopore with illumina whole genome assemblies of the Epstein-Barr virus in Burkitt lymphoma
    Article Snippet: .. While Illumina sequencing has significantly decreased in cost over the last several years, this pipeline makes ONT cheaper for sequencing EBV genomes, especially when taking into account the need for RNA capture baits in Illumina . ..

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Article Title: A population-based analysis of the molecular landscape of glioma in adolescents and young adults reveals insights into gliomagenesis.
    Article Snippet: Gliomas are a major cause of cancer-related deaths in adolescents and young adults (AYAs; ages 15–39 years).. Different molecular alterations drive gliomas in children and adults, leading to distinct biology and clinical consequences, but the implications of pediatricversus adult-type alterations in AYAs are unknown.. Our population-based analysis of 1,456 clinically and molecularly characterized gliomas in patients aged 0–39 years addresses this gap.

    Amplification:

    Article Title: eDNA Metabarcoding: A Novel Approach for Identifying Marine Animal Organism in Mandalika’s Coastal
    Article Snippet: Special Economic Zone (SEZ) Mandalika is an area designated by the Indonesian government as part of its strategy to accelerate development and boost economic growth in West Nusa Tenggara, specifically Central Lombok Regency.. Established under Government Regulation No. 52 of 2014, The SEZ covers approximately 1,035.67 Ha in Pujut District, on southern coast of Lombok Island, Indonesia.. Beyond it is reputation associated with the Mandalika Circuit, this area also known for it is marine tourism potential, Mandalika is characterized by it is pristine white sandy beaches and scenic landscapes surrounded by hills (National Council of Special Economic Zones 2015).

    Polymerase Chain Reaction:

    Article Title: eDNA Metabarcoding: A Novel Approach for Identifying Marine Animal Organism in Mandalika’s Coastal
    Article Snippet: Special Economic Zone (SEZ) Mandalika is an area designated by the Indonesian government as part of its strategy to accelerate development and boost economic growth in West Nusa Tenggara, specifically Central Lombok Regency.. Established under Government Regulation No. 52 of 2014, The SEZ covers approximately 1,035.67 Ha in Pujut District, on southern coast of Lombok Island, Indonesia.. Beyond it is reputation associated with the Mandalika Circuit, this area also known for it is marine tourism potential, Mandalika is characterized by it is pristine white sandy beaches and scenic landscapes surrounded by hills (National Council of Special Economic Zones 2015).

    Article Title: A population-based analysis of the molecular landscape of glioma in adolescents and young adults reveals insights into gliomagenesis.
    Article Snippet: Gliomas are a major cause of cancer-related deaths in adolescents and young adults (AYAs; ages 15–39 years).. Different molecular alterations drive gliomas in children and adults, leading to distinct biology and clinical consequences, but the implications of pediatricversus adult-type alterations in AYAs are unknown.. Our population-based analysis of 1,456 clinically and molecularly characterized gliomas in patients aged 0–39 years addresses this gap.

    In Vitro:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    FACS:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    DNA Sequencing:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Transduction:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Staining:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Flow Cytometry:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Luciferase:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Cytotoxicity Assay:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    In Vivo:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Immunohistochemistry:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Imaging:

    Article Title: Engineering sonogenetic EchoBack-CAR T cells.
    Article Snippet: In brief Sonogenetic EchoBack-CAR T cells were designed with an ultrasensitive heatshock promoter screened from a library and a synthetic positive feedback loop that reprograms tumor engagement into CAR T cell activation.. In this approach, a short burst of focused-ultrasound stimulation triggers long-lasting CAR T cell activation and continuous cancer cell destructionwhilemaintainingminimal toxicity.

    Plasmid Preparation:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Bioprocessing:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Mutagenesis:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Transfection:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Expressing:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Extraction:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Virus:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Binding Assay:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Neutralization:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Biomarker Discovery:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Recombinant:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..

    Purification:

    Article Title: Functional and antigenic landscape of the Nipah virus receptor-binding protein.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d METHOD DETAILS 12 B Data availability and interactive plots of results B Plasmid maps and primer sequences B Creation of CHO-bEFNB2 and CHO-bEFNB3 target cells B Anti-RBP monoclonal antibodies B Lentivirus backbone for RBP deep mutational scanning Cell 188, 1–15, May 1, 2025 B RBP mutant library design and production B Rescue of RBP pseudovirus library from transfection and creation of cell-stored mutant library B Rescue of RBP and F expressing pseudoviruses from cell-stored mutant library B Long-read sequencing to link mutations to barcodes B Extraction of lentiviral episomal DNA B Illumina barcode sequencing B Validations of DMS data using single mutant Nipah pseudoviruses B Production of a VSV-neutralization standard virus B Selections to determine effects of mutations on cell entry B Selections to determine effects of mutations on receptor binding B Selections to determine effects of mutations on antibody neutralization B Data filtering B Validation of RBP binding measurements by biolayer interferometry B Nipah virus RBP recombinant production B Monomeric Pteropus alecto Ephrin-B2 and -B3 purification B Pteropus alecto Ephrin B2-hFc and B3-hFc production B Logo plots B Structural analyses B Sequence analysis B Figures SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j.cell. ..



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    Genome visualization and annotation of strain LSDY01. (A) Chromosome; (B) plasmid. The element colour of each circle is indicated in the legend; (C) average Nucleotide Identity (ANI) heatmap illustrating genomic similarity among selected Bacillus strains. LSDY01 shows the highest ANI (99.6%) with Bacillus <t>licheniformis</t> ATCC 14580 and DSM 13, confirming its taxonomic assignment to this species. The color scale represents percentage similarity.
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    Genome visualization and annotation of strain LSDY01. (A) Chromosome; (B) plasmid. The element colour of each circle is indicated in the legend; (C) average Nucleotide Identity (ANI) heatmap illustrating genomic similarity among selected Bacillus strains. LSDY01 shows the highest ANI (99.6%) with Bacillus <t>licheniformis</t> ATCC 14580 and DSM 13, confirming its taxonomic assignment to this species. The color scale represents percentage similarity.
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    Adaptive Biotechnologies Corp b cell igh receptor sequencing
    Genome visualization and annotation of strain LSDY01. (A) Chromosome; (B) plasmid. The element colour of each circle is indicated in the legend; (C) average Nucleotide Identity (ANI) heatmap illustrating genomic similarity among selected Bacillus strains. LSDY01 shows the highest ANI (99.6%) with Bacillus <t>licheniformis</t> ATCC 14580 and DSM 13, confirming its taxonomic assignment to this species. The color scale represents percentage similarity.
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    Unrelated control (black), heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransfer to nitrocellulose, and immunoblotting <t>with</t> <t>anti-human</t> <t>proinsulin.</t> The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HET CORR n = 3, HET n = 4). ( C ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE, and the completed gel was then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents the shared marker and positive control. ( D ) Proinsulin and ( E ) insulin content (ng) normalized to total protein content (μg). ( F ) Proinsulin to insulin content ratio from ( D , E ). ( D – F ) HET CORR n = 4, HET n = 7. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .
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    Immune Epitope Database & Analysis Resource 208265 experimentally validated linear b cell epitope sequences
    Unrelated control (black), heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransfer to nitrocellulose, and immunoblotting <t>with</t> <t>anti-human</t> <t>proinsulin.</t> The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HET CORR n = 3, HET n = 4). ( C ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE, and the completed gel was then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents the shared marker and positive control. ( D ) Proinsulin and ( E ) insulin content (ng) normalized to total protein content (μg). ( F ) Proinsulin to insulin content ratio from ( D , E ). ( D – F ) HET CORR n = 4, HET n = 7. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .
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    Unrelated control (black), heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransfer to nitrocellulose, and immunoblotting <t>with</t> <t>anti-human</t> <t>proinsulin.</t> The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HET CORR n = 3, HET n = 4). ( C ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE, and the completed gel was then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents the shared marker and positive control. ( D ) Proinsulin and ( E ) insulin content (ng) normalized to total protein content (μg). ( F ) Proinsulin to insulin content ratio from ( D , E ). ( D – F ) HET CORR n = 4, HET n = 7. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .
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    Unrelated control (black), heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransfer to nitrocellulose, and immunoblotting <t>with</t> <t>anti-human</t> <t>proinsulin.</t> The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HET CORR n = 3, HET n = 4). ( C ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE, and the completed gel was then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents the shared marker and positive control. ( D ) Proinsulin and ( E ) insulin content (ng) normalized to total protein content (μg). ( F ) Proinsulin to insulin content ratio from ( D , E ). ( D – F ) HET CORR n = 4, HET n = 7. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .
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    Image Search Results


    Genome visualization and annotation of strain LSDY01. (A) Chromosome; (B) plasmid. The element colour of each circle is indicated in the legend; (C) average Nucleotide Identity (ANI) heatmap illustrating genomic similarity among selected Bacillus strains. LSDY01 shows the highest ANI (99.6%) with Bacillus licheniformis ATCC 14580 and DSM 13, confirming its taxonomic assignment to this species. The color scale represents percentage similarity.

    Journal: Frontiers in Microbiology

    Article Title: Genomic characterization of a pathogenic Bacillus licheniformis strain LSDY01: deciphering its genetic diversity and virulence-associated traits

    doi: 10.3389/fmicb.2026.1815181

    Figure Lengend Snippet: Genome visualization and annotation of strain LSDY01. (A) Chromosome; (B) plasmid. The element colour of each circle is indicated in the legend; (C) average Nucleotide Identity (ANI) heatmap illustrating genomic similarity among selected Bacillus strains. LSDY01 shows the highest ANI (99.6%) with Bacillus licheniformis ATCC 14580 and DSM 13, confirming its taxonomic assignment to this species. The color scale represents percentage similarity.

    Article Snippet: For comparative genomic analysis with Bacillus licheniformis , a total of 498 B. licheniformis genome sequences (October 2025) were retrieved from the National Center for Biotechnology Information (NCBI).

    Techniques: Plasmid Preparation

    Molecular typing and genomic phylogeny of Bacillus licheniformis strain LSDY01. (A) In silico multi-locus sequence typing (MLST) reveals that LSDY01 belongs to the uncommon ST20, differing from the prevalent ST3 by a single allele. (B) cgMLST Phylogenetic analysis places LSDY01 in a clade with Daqu-derived strains CP143961.1 and CP143962.1 (bootstrap = 100), indicating recent divergence.

    Journal: Frontiers in Microbiology

    Article Title: Genomic characterization of a pathogenic Bacillus licheniformis strain LSDY01: deciphering its genetic diversity and virulence-associated traits

    doi: 10.3389/fmicb.2026.1815181

    Figure Lengend Snippet: Molecular typing and genomic phylogeny of Bacillus licheniformis strain LSDY01. (A) In silico multi-locus sequence typing (MLST) reveals that LSDY01 belongs to the uncommon ST20, differing from the prevalent ST3 by a single allele. (B) cgMLST Phylogenetic analysis places LSDY01 in a clade with Daqu-derived strains CP143961.1 and CP143962.1 (bootstrap = 100), indicating recent divergence.

    Article Snippet: For comparative genomic analysis with Bacillus licheniformis , a total of 498 B. licheniformis genome sequences (October 2025) were retrieved from the National Center for Biotechnology Information (NCBI).

    Techniques: In Silico, Sequencing, Derivative Assay

    BRIG-based comparative genomic analysis of Bacillus licheniformis LSDY01. (A) Circular comparison of the LSDY01 chromosome with closely related strains CP143961.1 and CP143962.1 . A unique ~157 kb genomic island (position ~2.3–2.5 Mb) was identified, characterized by significantly lower GC content (33.03%) and the co-localization of an anti-CRISPR gene (AcrIIA7) and a yoqS locus. (B) Plasmid pLSDY01 alignment with reference sequences CP076292.1 and CP178437.1 , showing high identity (97.66%) and coverage (99%). The Pistol gene is uniquely present in pLSDY01. (C) Linear comparison of the ~157 kb genomic island with those of CP154901.1 and CP143961.1 . The gray shading indicates regions of shared homology among different elements. The open reading frames are marked by colored arrows.

    Journal: Frontiers in Microbiology

    Article Title: Genomic characterization of a pathogenic Bacillus licheniformis strain LSDY01: deciphering its genetic diversity and virulence-associated traits

    doi: 10.3389/fmicb.2026.1815181

    Figure Lengend Snippet: BRIG-based comparative genomic analysis of Bacillus licheniformis LSDY01. (A) Circular comparison of the LSDY01 chromosome with closely related strains CP143961.1 and CP143962.1 . A unique ~157 kb genomic island (position ~2.3–2.5 Mb) was identified, characterized by significantly lower GC content (33.03%) and the co-localization of an anti-CRISPR gene (AcrIIA7) and a yoqS locus. (B) Plasmid pLSDY01 alignment with reference sequences CP076292.1 and CP178437.1 , showing high identity (97.66%) and coverage (99%). The Pistol gene is uniquely present in pLSDY01. (C) Linear comparison of the ~157 kb genomic island with those of CP154901.1 and CP143961.1 . The gray shading indicates regions of shared homology among different elements. The open reading frames are marked by colored arrows.

    Article Snippet: For comparative genomic analysis with Bacillus licheniformis , a total of 498 B. licheniformis genome sequences (October 2025) were retrieved from the National Center for Biotechnology Information (NCBI).

    Techniques: Comparison, CRISPR, Plasmid Preparation

    Phylogenetic distribution of virulence-associated genes in Bacillus licheniformis strains. Maximum-likelihood tree of 336 Bacillus licheniformis genomes. Notably, yoqS, yoqJ , and Pistol were uniquely identified in strain LSDY01, while hrtA was present only in a subset of strains, highlighting the distinct gene profile of LSDY01 compared to other Bacillus licheniformis isolates.

    Journal: Frontiers in Microbiology

    Article Title: Genomic characterization of a pathogenic Bacillus licheniformis strain LSDY01: deciphering its genetic diversity and virulence-associated traits

    doi: 10.3389/fmicb.2026.1815181

    Figure Lengend Snippet: Phylogenetic distribution of virulence-associated genes in Bacillus licheniformis strains. Maximum-likelihood tree of 336 Bacillus licheniformis genomes. Notably, yoqS, yoqJ , and Pistol were uniquely identified in strain LSDY01, while hrtA was present only in a subset of strains, highlighting the distinct gene profile of LSDY01 compared to other Bacillus licheniformis isolates.

    Article Snippet: For comparative genomic analysis with Bacillus licheniformis , a total of 498 B. licheniformis genome sequences (October 2025) were retrieved from the National Center for Biotechnology Information (NCBI).

    Techniques:

    Strong biofilm formation by Bacillus licheniformis LSDY01. Biofilm biomass (OD 570 ) was measured by crystal violet staining after 36 h at 37 °C in TSB with 1% glucose. LSDY01 (0.67 ± 0.03) was classified as a strong producer. Error bars: SD of quadruplicate wells.

    Journal: Frontiers in Microbiology

    Article Title: Genomic characterization of a pathogenic Bacillus licheniformis strain LSDY01: deciphering its genetic diversity and virulence-associated traits

    doi: 10.3389/fmicb.2026.1815181

    Figure Lengend Snippet: Strong biofilm formation by Bacillus licheniformis LSDY01. Biofilm biomass (OD 570 ) was measured by crystal violet staining after 36 h at 37 °C in TSB with 1% glucose. LSDY01 (0.67 ± 0.03) was classified as a strong producer. Error bars: SD of quadruplicate wells.

    Article Snippet: For comparative genomic analysis with Bacillus licheniformis , a total of 498 B. licheniformis genome sequences (October 2025) were retrieved from the National Center for Biotechnology Information (NCBI).

    Techniques: Staining

    Effect of Bacillus licheniformis LSDY01 on HEK293 cell viability after 24 h co-culture. CCK-8 was added and absorbance (OD₄₅₀) was measured at the indicated time points. All values were normalized by subtracting the background signal of cell-free medium. Data are presented as mean ± SD ( n = 3). Statistical significance was determined by independent samples t -test (two-tailed). At the 2 h, 4 h and 6 h readings, no significant differences were observed between the bacterial co-culture group and the control group ( p > 0.05 for all).

    Journal: Frontiers in Microbiology

    Article Title: Genomic characterization of a pathogenic Bacillus licheniformis strain LSDY01: deciphering its genetic diversity and virulence-associated traits

    doi: 10.3389/fmicb.2026.1815181

    Figure Lengend Snippet: Effect of Bacillus licheniformis LSDY01 on HEK293 cell viability after 24 h co-culture. CCK-8 was added and absorbance (OD₄₅₀) was measured at the indicated time points. All values were normalized by subtracting the background signal of cell-free medium. Data are presented as mean ± SD ( n = 3). Statistical significance was determined by independent samples t -test (two-tailed). At the 2 h, 4 h and 6 h readings, no significant differences were observed between the bacterial co-culture group and the control group ( p > 0.05 for all).

    Article Snippet: For comparative genomic analysis with Bacillus licheniformis , a total of 498 B. licheniformis genome sequences (October 2025) were retrieved from the National Center for Biotechnology Information (NCBI).

    Techniques: Co-Culture Assay, CCK-8 Assay, Two Tailed Test, Control

    Unrelated control (black), heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransfer to nitrocellulose, and immunoblotting with anti-human proinsulin. The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HET CORR n = 3, HET n = 4). ( C ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE, and the completed gel was then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents the shared marker and positive control. ( D ) Proinsulin and ( E ) insulin content (ng) normalized to total protein content (μg). ( F ) Proinsulin to insulin content ratio from ( D , E ). ( D – F ) HET CORR n = 4, HET n = 7. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .

    Journal: EMBO Molecular Medicine

    Article Title: A new form of diabetes caused by INS mutations defined by zygosity, stem cell and population data

    doi: 10.1038/s44321-025-00362-9

    Figure Lengend Snippet: Unrelated control (black), heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransfer to nitrocellulose, and immunoblotting with anti-human proinsulin. The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HET CORR n = 3, HET n = 4). ( C ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE, and the completed gel was then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused in Fig. as it represents the shared marker and positive control. ( D ) Proinsulin and ( E ) insulin content (ng) normalized to total protein content (μg). ( F ) Proinsulin to insulin content ratio from ( D , E ). ( D – F ) HET CORR n = 4, HET n = 7. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .

    Article Snippet: Mouse anti-human proinsulin B-C junction sequence KTRREAEDLQ , Abmart , Cat# B-C junction; RRID: AB_2921300.

    Techniques: Control, SDS Page, Electrotransfer, Western Blot, Transfection, Positive Control, Marker

    Unrelated control (black), homozygous R6C (HOM, pink) and isogenic corrected (HOM CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransferred to nitrocellulose, and immunoblotted with anti-human proinsulin. The blot was cropped and rearranged for clarity. The left panel is reused as in Fig. , as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HOM CORR n = 3, HOM n = 8) and ( C ) of homozygous R6C iPSC-β cells treated with vehicle DMSO or MG132 (10 μM) for 30 min ( n = 4). ( D ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE and the completed gel then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused as in Fig. as it represents a shared marker and a positive control. ( E ) Proinsulin and ( F ) insulin content normalized to total protein content. ( G ) Proinsulin to insulin content ratio from ( E , F ). ( E – G ) HOM CORR n = 8, HOM n = 12–13. ( H ) Insulin content normalized to total protein content along stage 7 (S7, HOM CORR n = 5, HOM n = 12), 1 week (LC1W, HOM CORR n = 3, HOM n = 3), 2 weeks (LC2W, HOM CORR n = 3, HOM n = 3), 3 weeks (LC3W, HOM CORR n = 3, HOM n = 3), and 4 weeks (LC4W) of long culture (LC, HOM CORR n = 12, HOM n = 15). All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .

    Journal: EMBO Molecular Medicine

    Article Title: A new form of diabetes caused by INS mutations defined by zygosity, stem cell and population data

    doi: 10.1038/s44321-025-00362-9

    Figure Lengend Snippet: Unrelated control (black), homozygous R6C (HOM, pink) and isogenic corrected (HOM CORR, blue) iPSCs were differentiated into β cells. ( A ) iPSC-islet lysates were analyzed by SDS-PAGE under reducing conditions, electrotransferred to nitrocellulose, and immunoblotted with anti-human proinsulin. The blot was cropped and rearranged for clarity. The left panel is reused as in Fig. , as it represents a shared control. ( B ) Quantification of preproinsulin to proinsulin ratio from ( A ) (unrelated control n = 3, HOM CORR n = 3, HOM n = 8) and ( C ) of homozygous R6C iPSC-β cells treated with vehicle DMSO or MG132 (10 μM) for 30 min ( n = 4). ( D ) iPSC-islet lysates were resolved by nonreducing 12%-NuPAGE and the completed gel then treated with 100 mM DTT at 60 °C for 10 min before electrotransfer to nitrocellulose and immunoblotting with anti-proinsulin. n = 2. Medium from Min6 β cells transfected with human proinsulin was used as a positive control (INS, lane next to marker, M). The blot was cropped and rearranged for clarity. The left panel is reused as in Fig. as it represents a shared marker and a positive control. ( E ) Proinsulin and ( F ) insulin content normalized to total protein content. ( G ) Proinsulin to insulin content ratio from ( E , F ). ( E – G ) HOM CORR n = 8, HOM n = 12–13. ( H ) Insulin content normalized to total protein content along stage 7 (S7, HOM CORR n = 5, HOM n = 12), 1 week (LC1W, HOM CORR n = 3, HOM n = 3), 2 weeks (LC2W, HOM CORR n = 3, HOM n = 3), 3 weeks (LC3W, HOM CORR n = 3, HOM n = 3), and 4 weeks (LC4W) of long culture (LC, HOM CORR n = 12, HOM n = 15). All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. .

    Article Snippet: Mouse anti-human proinsulin B-C junction sequence KTRREAEDLQ , Abmart , Cat# B-C junction; RRID: AB_2921300.

    Techniques: Control, SDS Page, Electrotransfer, Western Blot, Transfection, Positive Control, Marker

    Heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into long-cultured β cells. ( A – C ) Static proinsulin and insulin secretion in response to 2.8 mM glucose (G2.8), 16.8 mM glucose (G16.8), or 16.8 mM glucose plus 10 μM forskolin (G16.8 + Fk). HET CORR n = 4, HET n = 7. ( A ) Proinsulin and ( B ) insulin secretion (ng) normalized to protein content (μg). ( C ) Proinsulin to insulin ratio from ( A , B ). ( D – H ) Dynamic insulin secretion upon perifusion with 2.8 mM glucose, 16.8 mM glucose (G16.8), G16.8 plus exendin-4 (Ex4, 50 ng/mL, 11.8 nM) or G2.8 plus KCl (30 mM). HET CORR n = 3, HET n = 4. ( D ) Insulin secretion normalized to protein content, with ( E – H ) area under the curve (AUC) per minute of secretion at G2.8, G16.8, G16.8 + Ex4, and G2.8 + KCl. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. In time course line plots, data are shown as mean ± s.e.m. .

    Journal: EMBO Molecular Medicine

    Article Title: A new form of diabetes caused by INS mutations defined by zygosity, stem cell and population data

    doi: 10.1038/s44321-025-00362-9

    Figure Lengend Snippet: Heterozygous R6C (HET, yellow) and isogenic corrected (HET CORR, blue) iPSCs were differentiated into long-cultured β cells. ( A – C ) Static proinsulin and insulin secretion in response to 2.8 mM glucose (G2.8), 16.8 mM glucose (G16.8), or 16.8 mM glucose plus 10 μM forskolin (G16.8 + Fk). HET CORR n = 4, HET n = 7. ( A ) Proinsulin and ( B ) insulin secretion (ng) normalized to protein content (μg). ( C ) Proinsulin to insulin ratio from ( A , B ). ( D – H ) Dynamic insulin secretion upon perifusion with 2.8 mM glucose, 16.8 mM glucose (G16.8), G16.8 plus exendin-4 (Ex4, 50 ng/mL, 11.8 nM) or G2.8 plus KCl (30 mM). HET CORR n = 3, HET n = 4. ( D ) Insulin secretion normalized to protein content, with ( E – H ) area under the curve (AUC) per minute of secretion at G2.8, G16.8, G16.8 + Ex4, and G2.8 + KCl. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. In time course line plots, data are shown as mean ± s.e.m. .

    Article Snippet: Mouse anti-human proinsulin B-C junction sequence KTRREAEDLQ , Abmart , Cat# B-C junction; RRID: AB_2921300.

    Techniques: Cell Culture

    Homozygous R6C (HOM, pink) and isogenic corrected (HOM CORR, blue) iPSCs were differentiated into long-cultured β cells. ( A – C ) Static proinsulin and insulin secretion in response to 2.8 mM glucose (G2.8), 16.8 mM glucose (G16.8), or 16.8 mM glucose plus 10 μM forskolin (G16.8 + Fk). HOM CORR n = 8, HOM n = 12 (for proinsulin), HOM n = 13 (for insulin). ( A ) Proinsulin and ( B ) insulin secretion normalized to protein content. ( C ) Proinsulin to insulin ratio from ( A , B ). ( D – I ) Dynamic insulin secretion upon perifusion with 2.8 mM glucose, 16.8 mM glucose (G16.8), G16.8 plus exendin-4 (Ex4, 50 ng/mL, 11.8 nM), or G2.8 plus KCl (30 mM). HOM CORR n = 12, HOM n = 14. ( D ) Insulin secretion normalized to protein content, with ( E ) zoom in on 16.8 mM glucose response. ( F – I ) Area under the curve (AUC) per minute of secretion at G2.8, G16.8, G16.8 + Ex4 and G2.8 + KCl. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. In time course line plots, data are shown as mean ± s.e.m. .

    Journal: EMBO Molecular Medicine

    Article Title: A new form of diabetes caused by INS mutations defined by zygosity, stem cell and population data

    doi: 10.1038/s44321-025-00362-9

    Figure Lengend Snippet: Homozygous R6C (HOM, pink) and isogenic corrected (HOM CORR, blue) iPSCs were differentiated into long-cultured β cells. ( A – C ) Static proinsulin and insulin secretion in response to 2.8 mM glucose (G2.8), 16.8 mM glucose (G16.8), or 16.8 mM glucose plus 10 μM forskolin (G16.8 + Fk). HOM CORR n = 8, HOM n = 12 (for proinsulin), HOM n = 13 (for insulin). ( A ) Proinsulin and ( B ) insulin secretion normalized to protein content. ( C ) Proinsulin to insulin ratio from ( A , B ). ( D – I ) Dynamic insulin secretion upon perifusion with 2.8 mM glucose, 16.8 mM glucose (G16.8), G16.8 plus exendin-4 (Ex4, 50 ng/mL, 11.8 nM), or G2.8 plus KCl (30 mM). HOM CORR n = 12, HOM n = 14. ( D ) Insulin secretion normalized to protein content, with ( E ) zoom in on 16.8 mM glucose response. ( F – I ) Area under the curve (AUC) per minute of secretion at G2.8, G16.8, G16.8 + Ex4 and G2.8 + KCl. All panels: Unpaired t -test. In box plots, the median of independent experiments is shown by a horizontal line; 25 th and 75 th percentiles are at the bottom and top of the boxes; whiskers represent the minimum and maximum values. In time course line plots, data are shown as mean ± s.e.m. .

    Article Snippet: Mouse anti-human proinsulin B-C junction sequence KTRREAEDLQ , Abmart , Cat# B-C junction; RRID: AB_2921300.

    Techniques: Cell Culture